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  • Title: VP4 typing of bovine and porcine group A rotaviruses by PCR.
    Author: Gouvea V, Santos N, Timenetsky Mdo C.
    Journal: J Clin Microbiol; 1994 May; 32(5):1333-7. PubMed ID: 8051262.
    Abstract:
    A PCR typing assay was developed to identify rotavirus P types (VP4 specificity) of bovine NCDV, UK, and B223 strains and porcine OSU and Gottfried strains. Thirty-nine human and animal strains representing all known, and some undefined, rotavirus P types were used to develop and evaluate the specificity of the method. No cross-amplification was observed. The PCR results agreed with previous characterizations by monoclonal antibodies, sequence analysis, and hybridization assays, except for the Gottfried strain, which showed a P type distinct from the human asymptomatic strains. Analysis of a small number of field specimens suggested a high level of VP4 polymorphism among porcine strains. The assay should be of value in typing field isolates and tracing interspecies infections.
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