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  • Title: The effect of three cryoprotective diluents on the in vitro transcription of ram sperm cells.
    Author: Miteva K, Goncharova-Peinova I, Zlatarev S, Pironcheva G, Russev G.
    Journal: Cytobios; 1994; 77(311):203-5. PubMed ID: 8055705.
    Abstract:
    The effect of three different solvents was investigated in run-on experiments, using purified ram sperm nuclei. The best cryoprotective diluent proved to be Nagase-Niwa, which had the most positive effect on transcription. The kinetic data of incorporation of 32P-ATP in pre-mRNA indicated that there was a stepwise increase in the level of transcription during the first 20 min of incubation, being lowest in the control and highest in the presence of the diluent Nagase-Niwa.
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