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  • Title: A method for protein assay in Laemmli buffer.
    Author: Karlsson JO, Ostwald K, Kåbjörn C, Andersson M.
    Journal: Anal Biochem; 1994 May 15; 219(1):144-6. PubMed ID: 8059941.
    Abstract:
    A simple, cheap, and rapid assay for soluble and membrane-bound protein solubilized in Laemmli sodium dodecyl sulfate sample buffer is described. Following the addition of trichloroacetic acid to a final concentration of 24%, the induced turbidity was measured at 570 nm in a microplate reader, after an incubation period of approximately 10-30 min. The assay is suitable for measuring bovine serum albumin at a concentration of 15 to 500 micrograms/ml in Laemmli buffer, corresponding to 2 to 75 micrograms protein per well on the microtiter plate. Other nonproteinaceous macromolecules in the sample may introduce an error.
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