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  • Title: A comparative evaluation of three transfection procedures as assessed by resistance to G418 conferred to HEPG2 cells.
    Author: Kumar S, O'Dowd C, Dunckley MG, Lund T.
    Journal: Biochem Mol Biol Int; 1994 Apr; 32(6):1059-66. PubMed ID: 8061622.
    Abstract:
    Three commonly used transfection techniques (electroporation, calcium phosphate precipitation and scrape loading) and a novel procedure combining the latter two methods were evaluated and conditions optimised for successful transfection of human HepG2 cells with plasmid DNA incorporating a mouse MHC Class I gene and a selectable marker (neomycin transferase gene) conferring resistance to G418. While transfection with linear DNA by scrape-loading gave satisfactory results, transfer of cloned circular DNA by electroporation, calcium phosphate precipitation or a combined use of scrape-loading and calcium phosphate gave best results for HepG2 cells.
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