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  • Title: Expression and crystallization of a soluble form of Drosophila fasciclin III.
    Author: Strong RK, Vaughn DE, Bjorkman PJ, Snow PM.
    Journal: J Mol Biol; 1994 Aug 19; 241(3):483-7. PubMed ID: 8064861.
    Abstract:
    A truncated form of Drosophila fasciclin III has been engineered by site-directed mutagenesis. Secreted fasciclin III is expressed at 35 to 40 mg/l in insect cells with baculovirus carrying the recombinant gene. Single crystals of purified soluble fasciclin III have been grown by vapor diffusion versus polyethylene glycol 8000/sodium citrate at low pH. The space group is P6(1)22 or its enantiomorph P6(5)22, with unit cell dimensions a = b = 140 A, c = 260 A. Cryo-preserved crystals diffract to reciprocal lattice spacings beyond 3.0 A.
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