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  • Title: Relative quantitative assay of the biological activity of interferon messenger ribonucleic acid.
    Author: Kronenberg LH, Friedmann T.
    Journal: J Gen Virol; 1975 May; 27(2):225-38. PubMed ID: 806662.
    Abstract:
    RNA extracted from cells previously stimulated to synthesize the antiviral protein, interferon, causes species-specific interferon synthesis when added to heterospecific cell cultures. Our results confirm the report of De Maeyer-Geugnard, De Maeyer & Montagnier (1972). We have used this observation to obtain a relative quantitative assay for the interferon messenger RNA activity. At appropriate RNA concentrations, the yield of interferon is proportional to the concentration of RNA adsorbed to recipient cell cultures.
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