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  • Title: Monoclonal antibody to proteoglycan derived from Grifola frondosa (Maitake).
    Author: Hirata A, Adachi Y, Itoh W, Komoda M, Tabata K, Sugawara I.
    Journal: Biol Pharm Bull; 1994 Apr; 17(4):539-42. PubMed ID: 8069265.
    Abstract:
    A murine monoclonal antibody (MAb) was prepared by immunizing BALB/c mice with a proteoglycan fraction derived from Grifola frondosa (Maitake mushroom), followed by the hybridization of spleen cells with mouse myeloma cells. The MAb (subclass; Ig G2b), designated MPG2, reacted with schizophyllan (SPG), curdlan, scleroglucan, laminarin and lentinan, but not with dextran, pullulan, mannan and xylan. Immunohistochemistry (ABC-GO method) showed that MAb MPG2 reacted with lysosomal proteoglycan and (1-->6)-beta-branched laminaritriose taken up by rabbit peritoneal macrophages. These results suggest that this MAb may recognize mainly (1-->3)-beta-D-glucan, and may be useful for determining the immunological properties of Grifola frondosa-derived proteoglycan.
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