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  • Title: Tetracycline resistance in Haemophilus influenzae is plasmid-linked.
    Author: Van AD, Bieth G, Bouanchaud DH.
    Journal: C R Acad Hebd Seances Acad Sci D; 1975 Mar 10; 280(10):1321-3. PubMed ID: 807346.
    Abstract:
    A strain of Haemophilus influenzae resistant to tetracycline (MIC 16-32 mug/ml) was "cured" of tetracycline resistance by ethidium bromide. DNA centrifugation in cesium chloride + ethidium bromide density gradients showed the existence of a band of non-chromosomal DNA molecules in the original resistant strain. This band was not found in lysates of a strain "cured" of tetracycline resistance by ethidium bromide. These facts support the hypothesis that in this strain of Haemohpilus influenzae, tetracycline resistance is plasmid mediated.
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