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  • Title: A new matrix for affinity chromatography and its application in the separation of a human monoclonal antibody.
    Author: Guo W, Shang Z, Yu Y, Zhou L.
    Journal: Biomed Chromatogr; 1994; 8(3):142-4. PubMed ID: 8075524.
    Abstract:
    A method for the preparation of a p-aminobenzene sulphonyl ethyl containing crosslinked Sepharose 4B (ABSE-Sepharose 4B-CL) is described, trypsin, bovine serum albumin (BSA) and concanavalin A (Con A) were immobilized onto this matrix by diazotization. Conditions for the coupling reaction were investigated. The activity of immobilized trypsin reached 1.4 x 10(4) U/g, and 25 mg BSA can be coupled onto 1 g ABSE-Sepharose 4B-CL under the optimal conditions. An affinity medium with immobilized Con A as ligand was prepared by this method, and was used in the separation of a human monoclonal antibody.
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