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  • Title: Characterization of monoclonal gammopathies, a new approach.
    Author: Nerenberg ST, Mallin J.
    Journal: J Lab Clin Med; 1975 Aug; 86(2):266-73. PubMed ID: 807664.
    Abstract:
    A new approach to the characterization (typing) of L- and H-chains of monoclonal immunoglobulins and Bence-Jones protein utilizing elution of a monoclonal immunoglobulin in conjunction with solid-support electrophoresis and the reverse Mancini procedure of radial immunodiffusion on Mylar-backed cellulose acetate membranes is described. A number of benefits are derived, particularly the use of only a fraction (1/200) of the antisera used with agar immunoelectrophoresis. The importance of "standardizing" commercial antisera (and a method for carrying this out) with the techniques described is emphasized.
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