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  • Title: Cloning, sequencing and overexpression of the Desulfovibrio gigas ferredoxin gene in E. coli.
    Author: Chen B, Menon NK, Dervertarnian L, Moura JJ, Przybyla AE.
    Journal: FEBS Lett; 1994 Sep 12; 351(3):401-4. PubMed ID: 8082803.
    Abstract:
    We have cloned the gene encoding Desulfovibrio gigas ferredoxin using a photodigoxigenin-labelled probe synthesized with the polymerase chain reaction. The DNA sequence of the gene predicts a polypeptide of 58 residues after removal of the initial formyl methionine (polypeptide M(r) = 6,276). The ferredoxin gene was expressed in aerobically grown E. coli behind the lac promoter of pUC18 resulting in a high level of ferredoxin expression which comprises about 10% of the total cell protein. EPR analysis of recombinant ferredoxin revealed the presence of a [3Fe-4S] cluster which is characteristic of native D. gigas ferredoxin II.
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