These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Purification and characterization of two new c-type cytochromes involved in Fe2+ oxidation from Thiobacillus ferrooxidans.
    Author: Valkova-Valchanova MB, Chan SH.
    Journal: FEMS Microbiol Lett; 1994 Aug 01; 121(1):61-9. PubMed ID: 8082827.
    Abstract:
    Two new c-type cytochromes have been purified from cell membranes of the acidophilic Thiobacillus ferrooxidans. In contrast to a soluble cytochrome c with molecular mass of 14 kDa reported earlier, a membrane-bound cytochrome c with a mass of 21 kDa was solubilized with octylthioglucoside and purified to homogeneity. In addition, a high molecular mass c-type cytochrome (68 kDa) was also solubilized and purified using Triton X-100 as a detergent. Both acid-stable species are partially released during osmotic shock and chloroform treatment of the bacteria; they are integral components in the respiratory chain donating electrons to the terminal cytochrome oxidase.
    [Abstract] [Full Text] [Related] [New Search]