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  • Title: Improved nuclear staining with mordant blue 3 as a hematoxylin substitute.
    Author: Llewellyn BD.
    Journal: Stain Technol; 1978 Mar; 53(2):73-7. PubMed ID: 80845.
    Abstract:
    For progressive staining 1 g mordant blue 3, 0.5 g iron a alum and 10 ml hydrochloric acid are combined to make 1 liter with distlled water. Paraffin sections are stained 5 minutes blued in 0.5% sodium acetate for 30 seconds and counterstained with eosin. For regressive staining, 1 g dye, 9 g iron alum and 50 ml acetic acid are combined to make 1 liter with distilled water. Staining time is 5 minutes followed by differentiation in 1% acid alcohol and blueing in 0.5% sodium acetate. Counterstain with eosin. In both cases results very closely results very resemble a good hematoxylin and eosin.
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