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  • Title: Evidence for posttranscriptional regulation of transgenic protein kinase C-alpha in T cells.
    Author: Iwamoto T, Ohkusu K, Watanabe M, Hidaka H, Nakashima I.
    Journal: J Cell Biochem; 1994 Jun; 55(2):264-71. PubMed ID: 8089201.
    Abstract:
    Recently, we succeeded in establishing a transgenic mouse line which expressed high levels of protein kinase C (PKC)-alpha in thymocytes at the mRNA level with disproportionately small increases at the protein level. The transgenic PKC-alpha was nevertheless functionally active for inducing accelerated cell growth and IL-2 production by stimulation with anti-receptor (CD3) antibody or phorbol 12-myristate 14-acetate (PMA) in vitro. Study of the dynamics of transgenic PKC-alpha in the cells in vitro showed that the amount of PKC-alpha protein increased in the cells remarkably at > or = 5 h after stimulation, whereas the level of PKC-alpha mRNA did not change significantly or changed slightly. This suggested that cell activation breaks the posttranscriptional regulation of the transgenic PKC-alpha in resting cells. The increase in PKC-alpha protein accompanied a prolonged membrane translocation of PKC-alpha and enhanced cell proliferation. Such a transgenic effect was inhibited completely by a PKC inhibitor, H-7, added during 0-6 h after the stimulation. These results show formally that the transgenic PKC-alpha whose production was accelerated through cell activation plays a key role in the late (for > or = 5 h) signal delivery for disregulated cell growth.
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