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  • Title: Effects of corticotrophin-releasing factor and arginine-vasopressin on proopiomelanocortin (POMC) mRNA levels, release and storage of adrenocorticotrophin from mouse anterior pituitary cells.
    Author: Castro MG.
    Journal: Comp Biochem Physiol Comp Physiol; 1993 Jan; 104(1):105-12. PubMed ID: 8094652.
    Abstract:
    1. The present studies were undertaken to determine the effects of arginine vasopressin (AVP), corticotrophin-releasing factor (CRF) and AVP in combination with CRF on proopiomelanocortin (POMC) gene expression, and in the release and biosynthesis of POMC products (i.e. ACTH). 2. After a 3 hr treatment, AVP (10(-7) M), CRF (10(-7) M) and AVP (10(-7) M) in combination with CRF (10(-7) M) stimulated ACTH release by 291 +/- 19.2%, 377.4 +/- 25.6% and 462.1 +/- 38.4% (P < 0.01), respectively, with respect to basal secretion; while ACTH content diminished by 84.0 +/- 4.6%, 81.3 +/- 2.1% and 71.0 +/- 1.5% (P < 0.01), respectively, with respect to basal. Total POMC mRNA levels were not affected after a 3 hr treatment. 3. When cells were treated with a wide range of AVP concentrations (10(-11)-10(-7) M) to which we added different concentrations of CRF, modulation of AVP-induced ACTH release was most effective at CRF concentrations of < 10(-10) M. 4. Prolonged (3-6 hr) exposure to as low as 10(-10) M CRF or 10(-9) M AVP resulted in homologous desensitization of ACTH secretion. However, these pretreated cells were able to respond to a further challenge of CRF and AVP. This paper provides new information on the dose ranges over which desensitization and cross-sensitization between CRF and AVP secretory effects take place in mouse anterior pituitary cells.
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