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  • Title: Enhanced binding to antigen of monoclonal antibodies modified with a crosslinker.
    Author: Ueno H, Masuko T, Wang J, Hashimoto Y.
    Journal: Biochem Biophys Res Commun; 1993 Mar 15; 191(2):701-8. PubMed ID: 8096384.
    Abstract:
    Modification of monoclonal antibodies (mAbs) with a photo-crosslinker, sulfosuccinimidyl 2-(m-azido-o-nitrobenzamido)-ethyl-1, 3'-dithiopropionate (SAND), enhanced their antigen binding activity. By SAND modification, SV2-61 gamma mAb against human c-erbB-2 protooncogene product could immunoprecipitate approximately an 8-fold greater amount of antigen, and AB-3 mAb against bovine serum albumin (BSA) bound to BSA more efficiently than unmodified mAb as determined by an enzyme-linked immunosorbent assay. Relative binding affinity of SAND-modified AB-3 was about 4-fold of unmodified AB-3. Augmented binding of mAbs was observed with or without photosensitization.
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