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  • Title: Cloning and restriction fragment length polymorphism analysis of a cDNA for swine PIT-1, a gene controlling growth hormone expression.
    Author: Tuggle CK, Yu TP, Helm J, Rothschild MF.
    Journal: Anim Genet; 1993 Feb; 24(1):17-21. PubMed ID: 8098921.
    Abstract:
    A partial swine cDNA which encodes the functional domain of PIT-1 was isolated by the polymerase chain reaction (PCR). The swine PIT-1 cDNA clone is 95% identical at the protein level to the rat Pit-1 gene. Thus, Pit-1's known function in control of rat growth hormone and prolactin expression is likely to be conserved in swine. This swine cDNA clone was used to investigate genetic variability at PIT-1 in several American and Chinese breeds. Polymorphic BamHI fragments were found in pure-bred Meishan animals (n = 13), but only monomorphic fragments in five American breeds (n = 36).
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