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  • Title: A simple method of characterizing mitochondrial ribonucleic acid.
    Author: Haldar D, Waters BK, Freeman DB.
    Journal: Biochem J; 1975 Jun; 147(3):621-3. PubMed ID: 810141.
    Abstract:
    A simple method of isolating and characterizing RNA from L-cell mitochondria is described. The mitochondrial fraction is lysed by sodium dodecyl sulphate, and the RNA fractionated by sucrose-density-gradient centrifugation. The efficacy of proteinase K in preventing ribonuclease activity is also demonstrated.
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