These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Identification of a 63-kDa serum protein that binds somatostatin and gastrin-releasing peptide but not bombesin.
    Author: Fanger BO, Wade AC, Cashman EA, Cardin AD.
    Journal: Biochim Biophys Acta; 1993 Nov 24; 1179(3):300-5. PubMed ID: 8105895.
    Abstract:
    A 63-kDa serum protein was identified that bound somatostatin and gastrin-releasing peptide (GRP) but not bombesin. The 63-kDa protein was detected by its ability to compete with the receptor for GRP in a receptor binding assay. This interaction could be inhibited by the addition of somatostatin, producing a higher and more accurate calculated affinity for the binding of GRP to its receptor. Somatostatin did not affect the affinity of the receptor for bombesin. Specificity of the 63-kDa protein for analogs of somatostatin, GRP, and bombesin was determined by competition with 125I-GRP and 125I-somatostatin. A tripeptide motif consisting of an aryl-hydrophobic-basic amino-acid structure in residues 15-17 of GRP (Tyr-Pro-Arg) and residues 7-9 of somatostatin (Phe-Trp-Lys) was implicated in binding. This tripeptide binding motif is not present in bombesin. That residues 15-17 of GRP are highly conserved suggests that its interaction with the 63 kDa serum protein may be physiological.
    [Abstract] [Full Text] [Related] [New Search]