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  • Title: Ultrathin-layer zone electrophoresis of lactate dehydrogenase isoenzymes in microdissected liver samples.
    Author: Maly IP, Toranelli M.
    Journal: Anal Biochem; 1993 Nov 01; 214(2):379-88. PubMed ID: 8109724.
    Abstract:
    A highly sensitive electrophoretic separation of lactate dehydrogenase isoenzymes in 150-microns ultrathin-layer polyacrylamide gels is described. By means of the incorporation of miniature-sized wells into the gel and by executing all the analytical steps under paraffin oil, this technique allows the exact and sharp separation of the lactate dehydrogenase isoenzymes in nanogram-sized microdissected liver tissue samples. With this method the heterogeneous distribution pattern of lactate dehydrogenase isoenzymes in the liver acinus of various mammals could be demonstrated, and a new interpretation of their functional roles is offered.
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