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  • Title: Improvement of culture conditions for L-proline production by a recombinant strain of Serratia marcescens.
    Author: Masuda M, Takamatu S, Nishimura N, Komatsubara S, Tosa T.
    Journal: Appl Biochem Biotechnol; 1993 Dec; 43(3):189-97. PubMed ID: 8109960.
    Abstract:
    Serratia marcescens SP511 was previously reported to be an L-proline-producing strain that harbors a recombinant plasmid carrying the mutant type of the proline operon. This strain produced 65 g/L of L-proline in a medium containing 22% sucrose and urea after 5 d of incubation under the conventional culture conditions. We searched for more suitable culture conditions for more abundant L-proline production by SP511. To improve the supply of a nitrogen source to cells, ammonium was used instead of urea and fed to a culture under control of the pH of the medium. The concentrations of MgSO4 and K2HPO4 were increased, and in addition, sucrose was continuously added to the culture at a final concentration of 32%. Under these conditions, the cell amount was increased twofold over that under the previous conditions and L-proline production reached a maximum of more than 100 g/L after 4 d of incubation.
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