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  • Title: Assignment of human satellite 1 DNA as revealed by fluorescent in situ hybridization with oligonucleotides.
    Author: Tagarro I, Wiegant J, Raap AK, González-Aguilera JJ, Fernández-Peralta AM.
    Journal: Hum Genet; 1994 Feb; 93(2):125-8. PubMed ID: 8112734.
    Abstract:
    We have used a fluorescent in situ hybridization procedure to detect human satellite 1 DNA, the simple sequence family that constitutes the non-male-specific fraction of classical satellite 1 DNA. Satellite 1 appears to be located on pericentromeric regions of chromosomes 3, 4 and 13, and on satellites of each acrocentric chromosome. These results suggest a possible relationship between quinacrine fluorescence of heterochromatin and DNA composition. Furthermore, by means of multicolour in situ hybridization, we have spatially resolved satellite 1 sequences and centromeric alpha-satellite within heterochromatic blocks.
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