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  • Title: An analysis of actin isoforms expressed in hair-cell enriched fractions of the chick basilar papilla by the polymerase chain reaction technique.
    Author: Pickles JO.
    Journal: Hear Res; 1993 Dec; 71(1-2):225-9. PubMed ID: 8113140.
    Abstract:
    Actin mRNA was characterised in hair-cell enriched fractions of the chick basilar papilla, by means of the reverse-transcription polymerase chain reaction technique. Primers were directed against the 3' untranslated portions of the actin mRNAs. Evidence for beta-cytoplasmic and gamma-cytoplasmic actin mRNA was found; no evidence was found for alpha-skeletal, alpha-cardiac or type 5 cytoplasmic actin mRNAs. Since beta-actin is known to form bundles of filaments whereas gamma-actin does not, this suggests that the hair-cell stereocilia are composed of beta-actin.
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