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  • Title: Protein-binding DNA regions inside and in the vicinity of the cytochrome P450bm-3 gene from Bacillus megaterium.
    Author: Gaidamakova EK, Karginov VA, Kovalenko SP.
    Journal: Biochem Biophys Res Commun; 1994 Feb 15; 198(3):862-8. PubMed ID: 8117290.
    Abstract:
    Protein-binding DNA-regions inside and in the vicinity of the phenobarbital-inducible P450bm-3 gene from Bacillus megaterium were characterized by gel-retardation technique and foot-printing analysis. Regions with induction-dependent protein binding capacity were shown to be situated in -279bp/-215bp, -215bp/+83bp and +236bp/+425bp fragments. Precise localization of DNA-protein contacts was established by foot-printing analysis for region -215bp/+83bp. The data obtained are discussed in line with previously reported information on the regulatory regions of various phenobarbital-regulated genes.
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