These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: A rapid and simple method to isolate and characterize highly polymorphic markers from the centromeric regions of the human chromosomes.
    Author: Laurent AM, Marçais B, Muleris M, Roizès G.
    Journal: Nucleic Acids Res; 1994 Jan 25; 22(2):194-9. PubMed ID: 8121804.
    Abstract:
    Using oligonucleotide primers complementary to the 3' ends of either the Alu or the L1Hs consensus sequences in conjunction with a primer complementary to alpha satellite subsets specific to different human chromosomes, it was possible to detect and characterize polymorphisms originating from the microsatellites which are often present downstream these repetitive elements. The methodology does not require cloning, sequencing or synthesis of specific primers. Centromeric location was confirmed by linkage analysis, in situ hybridization and sequencing. The method is proposed for the generation of polymorphic markers from all centromeric regions.
    [Abstract] [Full Text] [Related] [New Search]