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  • Title: A long-lasting vitamin C derivative, ascorbic acid 2-phosphate, increases myogenin gene expression and promotes differentiation in L6 muscle cells.
    Author: Mitsumoto Y, Liu Z, Klip A.
    Journal: Biochem Biophys Res Commun; 1994 Feb 28; 199(1):394-402. PubMed ID: 8123041.
    Abstract:
    Hormones and growth factors are important regulators of myogenic cell differentiation, but little is known about the effect of vitamins on muscle differentiation and development. We recently showed that L-ascorbic acid 2-phosphate, a stable form of vitamin C, increased the expression of muscle-specific glucose and ion transporters. We now show the effect of L-ascorbic acid 2-phosphate on the kinetics of myogenin expression at both the mRNA and protein levels during differentiation of L6 muscle cells. At the fully differentiated stage, control and L-ascorbic acid 2-phosphate treated cultures showed the same degree of cell fusion, but L-ascorbic acid 2-phosphate treated myotubes had a larger diameter than control myotubes. During L6 cell differentiation, the amount of both myogenin mRNA and protein reached a maximal level on day 4 before full myotube formation and then declined. L-ascorbic acid 2-phosphate treated cells expressed a higher amount of myogenin at both the mRNA and protein levels on day 4 compared to untreated cultures. Ethyl-3,4-dihydroxybenzoate, an inhibitor of collagen synthesis, prevented expression of myogenin mRNA and protein in both the control and L-ascorbic acid 2-phosphate treated cells. These results demonstrate that vitamin C can promote muscle differentiation likely through the increase of myogenin expression in myogenic cells, which may in turn regulate muscle differentiation in vivo.
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