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  • Title: Process monitoring of the production of gamma-interferon in recombinant Chinese hamster ovary cells.
    Author: Nadler TK, Paliwal SK, Regnier FE, Singhvi R, Wang DI.
    Journal: J Chromatogr A; 1994 Jan 28; 659(2):317-20. PubMed ID: 8124472.
    Abstract:
    The production of recombinant gamma-interferon was monitored using high-performance liquid chromatographic methods. These methods were able to distinguish between glycosylated and non-glycosylated forms of gamma-interferon by complexing the carbohydrate with borate. Sufficient quantities of standard glycosylated gamma-interferon were not available for peak identification so immunological techniques were used to identify gamma-interferon variants. These techniques were validated with the non-glycosylated form. The non-glycosylated form was then shown to be retained only on a cation-exchange column, while the glycosylated form, complexed with borate, was retained only on an anion-exchange column. Samples were drawn at 2-h intervals over a 60-h production cycle and analyzed by both anion- and cation-exchange chromatography. Results indicated that the production of each form was coincidental and that the glycosylated form of gamma-interferon is produced in greater abundance than non-glycosylated.
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