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  • Title: [Rifampicin: rabbits sensitization, two rifampicin-protein conjugates and detection of rifampicin specific IgG].
    Author: Li LF, Huang P.
    Journal: Zhonghua Yi Xue Za Zhi; 1993 Nov; 73(11):670-3, 702. PubMed ID: 8124616.
    Abstract:
    The presence of rifampicin (RFP) specific IgG in the sera of rabbits immunized with RFP or with two RFP-protein conjugates (RFP-bovine serum albumin, RFP-BSA and RFP-rabbit serum albumin RFP-RSA) was measured by ELISA. The way of conjugation and the molecular conjugation rate were different between RFP-BSA and RFP-RSA. The titre of RFP specific IgG was 1:10-1:100 in RFP immunized rabbits, 1:100-1:1,000 in RFP-RSA immunized rabbits, and over 1:1000 in RFP-BSA immunized rabbits. The binding properties of the sera to RFP were demonstrated by inhibition assay. These are the models of RFP sensitization first established by immunization animals with RFP itself or RFP-protein conjugates.
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