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  • Title: Development of a bispecific monoclonal antibody for use in molecular hybridisation.
    Author: Auriol J, Guesdon JL, Mazié JC, Nato F.
    Journal: J Immunol Methods; 1994 Feb 28; 169(1):123-33. PubMed ID: 8133070.
    Abstract:
    A mouse hybrid hybridoma (tetradoma) was prepared by fusing hybridomas producing monoclonal antibody to acetyl-aminofluorene with hybridomas producing antibody against calf intestine alkaline phosphatase. The tetradoma line established secreted immunoglobulin manifesting parental and bispecific binding characteristics. Bispecific monoclonal antibody was purified and used for a one-step immunodetection assay of non-radioactive DNA and RNA probes. The immunoassay developed was able to detect 5 pg DNA within 2 h and gave low background noise.
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