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  • Title: Site-directed mutagenesis of two highly conserved residues near the active site of phosphofructo-1-kinase.
    Author: Zheng RL, Kemp RG.
    Journal: Biochem Biophys Res Commun; 1994 Mar 15; 199(2):577-81. PubMed ID: 8135798.
    Abstract:
    Mutations of either of two highly conserved residues near the active site of Escherichia coli phosphofructokinase, Ile-126 or Asn-128, produce no changes in the Km for ATP, relatively small changes in kcat, and a large increase in the Km for fructose 6-P, despite the fact that these residues are not directly involved in substrate binding. A computer graphics analysis of the three-dimensional structure suggests that the mutations effect the orientation of Arg-252, a residue important both for fructose 6-P binding and for catalysis.
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