These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Multinuclear, multidimensional NMR studies of Anabaena 7120 heterocyst ferredoxin. Sequence-specific resonance assignments and secondary structure of the oxidized form in solution.
    Author: Chae YK, Abildgaard F, Mooberry ES, Markley JL.
    Journal: Biochemistry; 1994 Mar 22; 33(11):3287-95. PubMed ID: 8136364.
    Abstract:
    Sequence-specific assignments were determined for the diamagnetic proton resonances from recombinant Anabaena 7120 heterocyst ferredoxin (M(r) = 11,000) produced in Escherichia coli. Several samples selectively labeled with nitrogen-15 were prepared for use in two-dimensional heteronuclear multiple quantum coherence (HMQC) [Müller, L. (1979) J. Am. Chem. Soc. 101, 4481-4484] experiments. A sample uniformly labeled with nitrogen-15 was also prepared and used in two three-dimensional experiments: NOESY-HMQC and TOCSY-HMQC [Zuiderweg, E. R. P., & Fesik, S. W. (1989) Biochemistry 28, 2387-2391; Marion, D., Ikura, M., Tsuchudin, R., & Bax, A. (1989) J. Magn. Reson. 85, 393-399]. The sequential assignment strategy relied on the detection of 15N-edited interresidue 1H alpha i/1HNi+1 NOE connectivities. Starting points and checks were provided by HMQC spectra of the selectively labeled samples. A sample doubly labeled with carbon-13 and nitrogen-15 was also prepared and used in three triple-resonance experiments: HNCA, HNCO, and HN(CO)CA [Ikura, M., Kay, L. E., & Bax, A. (1990) Biochemistry 29, 4659-4667; Kay, L. E., Ikura, M., Tschudin, R., & Bax, A. (1990) J. Magn. Reson. 89, 496-514]. The HNCA and HN(CO)CA spectra, which were used to confirm assignments from NOE connectivities, provided independent sequential assignments from spin couplings. Resonances from 18 residues were not seen in the diamagnetic region of the NMR spectrum. Several of these residues are very close to the [2Fe-2S] cluster, and their absence is explained by paramagnetic broadening and/or shifting.(ABSTRACT TRUNCATED AT 250 WORDS)
    [Abstract] [Full Text] [Related] [New Search]