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  • Title: Aequorea green fluorescent protein. Expression of the gene and fluorescence characteristics of the recombinant protein.
    Author: Inouye S, Tsuji FI.
    Journal: FEBS Lett; 1994 Mar 21; 341(2-3):277-80. PubMed ID: 8137953.
    Abstract:
    Expression of the cDNA for Aequorea green fluorescent protein in E. coli yielded a fused protein with fluorescence excitation and emission spectra virtually identical to those of the native green fluorescent protein. Further, a solution of the protein, when mixed with aequorin and calcium ion, emitted a greenish luminescence characteristic of the in vivo luminescence of the animal, indicating a radiationless energy transfer to the protein.
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