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  • Title: Role of glutamate dehydrogenase reaction in the control of citrate pool in yeast.
    Author: Yoshino M, Murakami K.
    Journal: Int J Biochem; 1993 Dec; 25(12):1723-7. PubMed ID: 8138009.
    Abstract:
    1. Role of NADP-glutamate dehydrogenase in the depletion of citrate was analyzed using permeabilized yeast cells. 2. Citrate was converted to 2-oxoglutarate, which was then metabolized to glutamate by NADP-glutamate dehydrogenase in the presence of ammonium ion. 3. Formation of 2-oxoglutarate plus glutamate was in good agreement with the concentration of citrate decreased. Glutamate formation can be a good indicator of the depletion of citrate, because 70% of the citrate decreased was converted to glutamate. 4. Glycolytic activity was closely correlated with the decrease in citrate under the in situ conditions. 5. NADP-glutamate dehydrogenase increased in anaerobically grown yeast cells. 6. An effective depletion of citrate by increased synthesis of NADP-glutamate dehydrogenase can explain the lowered mechanism of citrate causing glycolytic stimulation under the anaerobic growth conditions of yeast.
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