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  • Title: Activation of osmotically-activated potassium transporters after injection of mRNA from A6 cells in Xenopus oocytes.
    Author: Ratcliff FG, Ehrenfeld J.
    Journal: Biochim Biophys Acta; 1994 Mar 23; 1190(2):248-56. PubMed ID: 8142423.
    Abstract:
    The different potassium pathways, under iso-osmotic or hypo-osmotic conditions, were examined in Xenopus oocytes that were micro-injected with mRNA from A6 cells. Hypo-osmotically stimulated 86Rb (K+) effluxes could be measured from intact oocytes 1-4 days after injection of 25 ng of poly (A)+ RNA isolated from A6 cells. 86Rb (K+) effluxes were 2.2 times higher from oocytes micro-injected with 25 ng of poly(A)+ RNA, than from water injected control oocytes. Water-injected oocytes themselves, however, were 7-fold more responsive to a hypo-osmotic shock than non-injected Xenopus oocytes. There was no significant effect of the different K+ transport blockers tested (TEA, bumetanide, glybenclamide or quinidine) on the endogenous 86Rb (K+) effluxes from non-injected oocytes in either iso- or hypo-osmotic media. The 86Rb (K+) effluxes from water-and mRNA-injected oocytes in hypo-osmotic media were both inhibited by TEA. In mRNA-injected oocytes the increase in 86Rb (K+) transport following a medium dilution was also inhibited in the presence of glybenclamide or bumetanide. The present study reports that the activation of hypo-osmotically-activated potassium transporters in the oocytes of Xenopus laevis. after injection of mRNA from A6 cells differs quantitatively and in part qualitatively (glybenclamide-sensitivity) from the endogenous K+ pathways of non-injected and of water-injected Xenopus oocytes.
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