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  • Title: The expression of PDGF-alpha but not PDGF-beta receptors is suppressed in Swiss/3T3 fibroblasts over-expressing protein kinase C-alpha.
    Author: Fitzer-Attas C, Eldar H, Eisenbach L, Livneh E.
    Journal: FEBS Lett; 1994 Apr 04; 342(2):165-70. PubMed ID: 8143871.
    Abstract:
    The generation and characterization of Swiss/3T3 cells which stably over-express protein kinase C (PKC)-alpha were previously described by us. In these cells over-expression of PKC-alpha reduced the expression of epidermal growth factor (EGF) receptor molecules [(1990) J. Biol. Chem. 265, 13290-13296]. Here we show that the expression of PDGF-alpha receptors, but not PDGF-beta receptors, was specifically decreased in these cells. Not only were the levels of PDGF-alpha receptor mRNA transcript and protein significantly diminished in the PKC-alpha over-producing cells, but their ability to respond to short- and long-term growth factor signals was appropriately compromised. This was reflected in a reduced tyrosine autophosphorylation signal in response to PDGF-AA, as well as in decreased growth rates of PKC-alpha over-expressing cells when supplied with external PDGF-AA. A similar decrease in PDGF-alpha receptors was also demonstrated in parental Swiss/3T3 cells treated with phorbol esters. Our studies imply that PKC-alpha is involved in a cellular mechanism suppressing the expression of PDGF-alpha receptors in Swiss/3T3 cells. Hence, activation of PKC-alpha or alterations in its cellular levels may affect, in turn, the expression of a specific set of cell surface receptors and their responses to external growth factors.
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