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  • Title: [Search for new products of gene expression in human cardiac muscle. Microsequencing of proteins after two-dimensional electrophoresis].
    Author: Laptev AV, Shishkin SS, Egorov TsA, Kovalev LI, Tsvetkova MN, Galiuk MA, Musaliamov AKh, Efimochkin AS.
    Journal: Mol Biol (Mosk); 1994; 28(1):52-8. PubMed ID: 8145754.
    Abstract:
    Proteins of the human heart muscle were studied using modified two-dimensional electrophoresis. After separation, proteins were electroblotted onto Immobilon P membranes and several protein spots were used for microsequencing analysis. In most cases the proteins analyzed have blocked N-terminal amino acids. In order to study the primary structure of these proteins, hydrolysis in situ by trypsin followed by reversed-phase HPLC and microsequencing of the resulting peptides were performed. Four protein were identified in 8 analyzed fractions, specifically myosin light chain 1 (MLCl-V/sB), fatty-acid binding protein (heart isoform), alpha (B)-crystallin and alpha-tropomyosin. Amino acid sequences of two proteins were not found among human amino acid sequences collected in SWISSPROT bank (v. 21).
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