These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Structural studies of endopolygalacturonase-resistant fragments of an antiulcer pectin from the roots of Bupleurum falcatum L.
    Author: Hirano M, Kiyohara H, Matsumoto T, Yamada H.
    Journal: Carbohydr Res; 1994 Jan 03; 251():145-62. PubMed ID: 8149369.
    Abstract:
    The endo-alpha-(1-->4)-polygalacturonase-resistant fractions (PG-1, PG-2, and PG-3) from an antiulcer pectin (Bupleuran 2IIc), isolated from the roots of Bupleurum falcatum L., were further analysed by lithium degradation. The results indicated that PG-1 contained a small proportion of long, branched arabinosyl chains and a large proportion of short, neutral oligosaccharide chains. GLC-MS analysis showed that, after methylation the short, neutral oligosaccharide fraction consisted of at least 22 kinds of di- to tetra-saccharide alditols, such as Gal-(1-->4)-Rha-ol (a major component), Ara-(1-->4)-Rha-ol, Glc-(1-->4)-Rha-ol, Ara-->Ara-->Ara-ol, and Ara-->Ara-->Ara-->Ara-ol (minor components) in addition to heteroglycosyl alditols. After deesterification, PG-2 and PG-3 were digested with endo-alpha-(1-->4)-polygalacturonase again, and the enzyme-resistant intermediate size fraction (PG-2') was purified. Component sugar analysis indicated that PG-2' contained 2-Me-Fuc, 2-Me-Xyl, apiose (Api), aceric acid (AceA), 3-deoxy-D-lyxo-heptulosaric acid (Dha), and 3-deoxy-D-manno-2-octulosonic acid (Kdo) in addition to Rha, Fuc, Ara, Xyl, Man, Gal, Glc, GalA, and GlcA. Lithium degradation of PG-2' gave mainly a pentosyl-->6-deoxyhexosyl-->6-deoxyhexosyl-->pentitol fragment, with some neutral di- and tri-saccharide alditols, including a pentosyl-->deoxyhexitol. Methylation analysis of these degradation products indicated that they contained terminal Rha, Araf, Fuc, Xyl, and Gal, 4-linked Rha, 3-linked Fuc, 3-linked Ara, and 3'-linked Api. Bupleuran 2IIc was eluted as essentially a single peak on gel filtration on Sepharose CL-6B. The neutral sugar content of the successive fractions increased with increasing molecular weight, but each fraction also contained, in addition to Rha, Ara, and Gal, 2-Me-Fuc, 2-Me-Xyl, and Api.
    [Abstract] [Full Text] [Related] [New Search]