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  • Title: HCO-3-activated adenosine triphosphatase in intestinal mucosa of the eel.
    Author: Morisawa M, Utida S.
    Journal: Biochim Biophys Acta; 1976 Sep 14; 445(2):458-63. PubMed ID: 8150.
    Abstract:
    An HCO-3-activated and SCN--inhibited ATPase (ATP phosphohydrolase, EC 3.6.1.3) found in homogenates of intestinal mucosa of the eel was solubilized by Triton X-100. Optimal HCO-3-concentration and pH for the enzyme were 25 mM and 8.7, respectively. HCO-3-ATPase activity in both homogenate and solubilized preparations increased after seawater adaptation. This adaptive increase in enzyme activity was also observed in the gills and the kidney. The HCO-3-ATPase seems to be related to transport mechanisms, especially for Cl-, in osmoregulatory surfaces of the eel.
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