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  • Title: A simple and rapid technique for the detection of rifampin resistance in Mycobacterium leprae.
    Author: Honoré N, Perrani E, Telenti A, Grosset J, Cole ST.
    Journal: Int J Lepr Other Mycobact Dis; 1993 Dec; 61(4):600-4. PubMed ID: 8151192.
    Abstract:
    The rifampin resistance of Mycobacterium leprae is due to missense mutations in the rpoB gene encoding the beta-subunit of the essential enzyme RNA polymerase. A rapid and very simple method has been developed to detect rifampin resistance in small numbers of M. leprae present in biopsies. It involves polymerase chain reaction amplification of a defined region of the rpoB gene followed by single-strand conformational polymorphism analysis (PCR-SSCP). The reliability of the method has been tested on a sample of known drug-resistant and susceptible isolates of M. leprae.
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