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  • Title: Differences in the acyl composition of the platelet-activating factor (PAF) precursor and other choline phosphoglycerides of the rabbit retinal rod outer segments and neural retina.
    Author: Bazan HE, Hurst JS, Bazan NG.
    Journal: Curr Eye Res; 1994 Jan; 13(1):45-50. PubMed ID: 8156825.
    Abstract:
    Choline phosphoglycerides comprise almost half of vertebrate retinal phospholipids. This lipid pool contains the precursor of the potent lipid mediator, platelet-activating factor. The acyl composition and distribution of the different subclasses of the choline phosphoglycerides (alkylacyl-[or the precursor of platelet-activating factor], alkenylacyl-[or choline plasmalogen] and diacyl-glycero-3-phosphocholine) were studied in intact rabbit retina, neural retina and rod outer segments. Choline phosphoglycerides were isolated by high performance liquid chromatography and derivatized by acetylation after phospholipase C treatment. The derivatives were purified by high performance liquid chromatography and subjected to methanolysis. Fatty acids were analyzed by capillary gas liquid chromatography. In the intact retina and in the neural retina, the alkylacyl-glycero-3-phosphocholine and alkenylacyl-glycero-3-phosphocholine comprise 1.2% and 1.5%, respectively, of the total choline phosphoglycerides, whereas the rod outer segments contain twice the proportion of the precursor of platelet-activating factor and no detectable plasmalogens. On a mole percent basis, arachidonic acid was highest in the neural retinal alkenylacyl-glycero-3-phosphocholine (27%), 18% in the alkylacyl-glycero-3-phosphocholine and only 5% in the diacyl-glycero-3-phosphocholine. However, alkylacyl-glycero-3-phosphocholine from rod outer segments was enriched in docosapentaenoic acid (18%) while arachidonic acid was in the 3-4% range. Our results suggest that, in the neural retina, alkyl-arachidonoyl-glycero-3-phosphocholine is a source of both platelet-activating factor and of arachidonic acid which may be a substrate for both prostaglandins and lipoxygenase metabolites during an inflammatory episode and may contribute to the retinal pathology.
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