These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


PUBMED FOR HANDHELDS

Search MEDLINE/PubMed


  • Title: Activity induced elevations of intracellular calcium concentration in neurons of the deep cerebellar nuclei.
    Author: Muri R, Knöpfel T.
    Journal: J Neurophysiol; 1994 Jan; 71(1):420-8. PubMed ID: 8158239.
    Abstract:
    1. Depolarization-induced changes in the cytosolic free calcium concentration ([Ca2+]i) were examined in slice-cultured neurons of the deep cerebellar nuclei by combined intracellular and multisite fura-2 recording techniques. 2. Firing of tetrodotoxin (TTX)-sensitive action potentials induced by depolarizing current pulses caused large elevations in somatic as well as proximal dendritic [Ca2+]i. In the dendrites, rise and decay times of [Ca2+]i were faster than in the soma. [Ca2+]i changes associated with depolarizations to < or = -40 mV in the presence of TTX were small compared with changes induced by Na+ spike firing, suggesting that Ca2+ influx through high voltage-activated Ca2+ channels is a major cause for Na+ spike-associated [Ca2+]i increases. 3. During sustained Na+ spike firing at a constant frequency (> 20 Hz), [Ca2+]i approached a constant level, after approximately 1 s in the dendrites and 2 s in the soma, respectively. The amplitude of the attained level was positively correlated with the firing frequency. We suggest that during tonic activity [Ca2+]i reaches a steady state determined by Ca2+ influx and extrusion. 4. TTX-resistant plateau potentials caused substantially greater [Ca2+]i increases in the dendrites than in the soma. In the dendrites, plateau-associated Ca2+ transients were comparable in amplitude to Ca2+ transients triggered by short (50 ms) Na+ spike trains, in the soma, they were considerably smaller. 5. Low-threshold spikes (LTSs) in association with a burst of Na+ spikes induced a sharp increase in [Ca2+]i both in the soma and in dendrites.(ABSTRACT TRUNCATED AT 250 WORDS)
    [Abstract] [Full Text] [Related] [New Search]