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  • Title: Fluidity of chicken ventricular plasma membranes during development in-ovo and after birth: spin labelling and fluorescence studies.
    Author: Daveloose D, Vezin H, Basse F, Viret J.
    Journal: J Mol Cell Cardiol; 1993 Dec; 25(12):1439-44. PubMed ID: 8158663.
    Abstract:
    The fluidity of isolated chicken ventricular plasma membranes was investigated by ESR spectroscopy, using three doxylstearic spin labels (5-SASL, 10-SASL and 16-SASL), and by fluorescence anisotropy measurements, using DPH probe. The analysis of 2T1 splitting constant and rotational correlation frequency have shown differences, during in-ovo development, characterized by a rigidification phase with a maximum at 19 days of development, just before hatching, followed by a fluidification phase after birth. These phenomena were also improved by DPH fluorescence and dynamical analysis of the hydrophobic 16-SASL probe.
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