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  • Title: HPLC determination of glutathione and L-cysteine in pharmaceuticals after derivatization with ethacrynic acid.
    Author: Di Pietra AM, Gotti R, Bonazzi D, Andrisano V, Cavrini V.
    Journal: J Pharm Biomed Anal; 1994 Jan; 12(1):91-8. PubMed ID: 8161611.
    Abstract:
    Ethacrynic acid and its methyl ester are proposed as useful pre-chromatographic derivatization reagents for the HPLC analysis (UV detection) of reduced glutathione (GSH) and L-cysteine. The optimum experimental conditions for the thiol derivatization, the removal of the excess reagent by liquid-liquid or solid-phase extraction and the reversed-phase chromatographic separations of the thiol adducts were investigated. The method was applied to the HPLC determination of GSH and L-cysteine in commercial formulations and proved to be suitable for the HPLC determination of oxidized glutathione (GSSG) after reduction to GSH using dithiothreitol (DTT).
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