These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [A new method of immobilizing proteolytic enzymes in polymeric hydrogels].
    Author: Markvicheva EA, Bronin AS, Kudriavtseva NE, Kuz'kina IF, Pashkin II, Kirsh IuE, Rumsh LD, Zubov VP.
    Journal: Bioorg Khim; 1994 Mar; 20(3):257-62. PubMed ID: 8166751.
    Abstract:
    A novel one-step method for enzyme immobilization in composite gels based on the thermally reversible polymer poly (N-vinyl-caprolactam) was developed. Conditions for entrapment of trypsin and carboxypeptidase B in the gels were optimized. After immobilization, 80 and 90% of the original carboxypeptidase B and trypsin activities, respectively, were found to retain. Both immobilizes enzymes were active in a wider pH and temperature range. The granules with entrapped enzymes were successfully applied to obtain human insulin from recombinant proinsulin.
    [Abstract] [Full Text] [Related] [New Search]