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  • Title: Use of lac gene fusions in the analysis of Acholeplasma upstream gene regulatory sequences.
    Author: Knudtson KL, Minion FC.
    Journal: J Bacteriol; 1994 May; 176(9):2763-6. PubMed ID: 8169231.
    Abstract:
    The objective of this study was to develop a means of identifying and analyzing mycoplasma gene regulatory elements. Analysis of Acholeplasma oculi ISM1499 transcriptional sequences was accomplished using a promoterless lacZ reporter gene and an integrative vector strategy. Seven promoter-containing fragments were identified, levels of beta-galactosidase were determined, and transcriptional start sites were mapped.
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