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  • Title: Inhibition of a K+ current by beta-dendrotoxin in primary and subcultured vascular smooth muscle cells.
    Author: Ren J, Karpinski E, Benishin CG.
    Journal: J Pharmacol Exp Ther; 1994 Apr; 269(1):209-14. PubMed ID: 8169827.
    Abstract:
    beta-Dendrotoxin (beta-DTX), a polypeptide component of Eastern Green Mamba snake venom, inhibits a slow voltage-activated 86Rb efflux from synaptosomes, suggesting that beta-DTX inhibits K+ channels. The effects of beta-DTX on the K+ currents in primary cultured and subcultured (passages 8-12) rat tail artery vascular smooth muscle cells (VSMCs) were studied using the whole-cell patch-clamp technique. A delayed rectifier K+ current was observed in both types of cells. The current, which was relatively insensitive to tetraethylammonium, was activated at -40 to -30 mV and showed almost no inactivation. beta-DTX (1-1000 nM) decreased the outward K+ current. The effect was concentration dependent and reversible by washout but did not depend on the frequency of stimulation (use dependence) or the membrane potential. beta-DTX was more effective in primary cultured cells than in subcultured cells. K+ channels in primary cultured cells were maximally (45%) inhibited by 1 microM beta-DTX compared with 35% inhibition in subcultured cells. The concentration producing half-maximal inhibition was 5.1 x 10(-8) M for primary cells and 7.1 x 10(-8) M for subcultured cells. The delayed rectifier current was not affected by alpha-DTX, a blocker of the fast-inactivating outward K+ current (IA). These results clearly demonstrate that beta-DTX is a novel antagonist of the delayed rectifier K+ current in primary and subcultured rat tail artery VSMCs.
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