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  • Title: Identification of a genomic fragment of Mycobacterium tuberculosis responsible for in vivo growth advantage.
    Author: Pascopella L, Collins FM, Martin JM, Jacobs WR, Bloom BR.
    Journal: Infect Agents Dis; 1993 Aug; 2(4):282-4. PubMed ID: 8173811.
    Abstract:
    The purpose of this work was to develop a system to identify virulence determinants of M. tuberculosis by genetic complementation. The ability to grow in mouse spleen and/or lung was defined as a potential phenotype for virulence. Enrichment for growing recombinant clones from a pool of H37Ra transformants containing the integrating pYUB178::H37Rv cosmid library was accomplished by in vivo selection. A molecular strategy was devised to isolate and clone the 25-kb H37Rv genomic fragment ivg that conferred in vivo growth advantage to H37Ra. This study is a first step toward understanding the genetics of virulence in M. tuberculosis. A detailed description of these experiments has been submitted for publication in Infection and Immunity.
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