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  • Title: Activated Gs alpha but not Gi alpha prevents the thermal inactivation of adenylyl cyclase in plasma membranes derived from S49 lymphoma cells.
    Author: Kvapil P, Novotny J, Ransnäs LA.
    Journal: FEBS Lett; 1994 May 02; 343(3):208-12. PubMed ID: 8174703.
    Abstract:
    The thermal inactivation of adenylyl cyclase was studied in plasma membranes isolated from wild-type and the mutant cell strain cyc- of S49 lymphoma. The half-life of adenylyl cyclase activity at 30 degrees C was decreased from 14.2 min to 3.4 min by the presence of detergents. ATP as well as forskolin prevented the adenylyl cyclase inactivation in a dose-response manner independent of the utilized type of cell membranes. Activation of G-proteins by GTP gamma S or by AlF-4 in wild-type membranes but not in cyc- membranes partially prevented adenylyl cyclase inactivation. Adenylyl cyclase activity in cyc- membranes was preserved in the presence of GTP gamma S or AlF-4 from the observed detergent-induced inactivation by complementation of these membranes with an extract from wild-type membranes. ADP-ribosylation of Gi alpha in cyc- membranes did not influence the kinetics of the inactivation process of adenylyl cyclase, whereas ADP-ribosylated Gs alpha protein protected adenylyl cyclase more effectively than non-ribosylated Gs alpha in wild-type plasma membranes when GTP was used as an activator.
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