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  • Title: Insulin-like growth factor 1 receptor-mediated endocytosis in Xenopus laevis oocytes. A role for receptor tyrosine kinase activity.
    Author: Taghon MS, Sadler SE.
    Journal: Dev Biol; 1994 May; 163(1):66-74. PubMed ID: 8174788.
    Abstract:
    Equilibrium analysis of [125I]IGF-1 binding to Xenopus oocytes yielded curvilinear Scatchards supporting the presence of multiple binding sites. High-affinity binding displayed an apparent KD of 9.0 +/- 3.0 nM IGF-1 with maximum total binding of 1.6 +/- 0.3 fmole/oocyte after 90 min at 22 degrees C. Binding of 1 nM [125I]IGF-1 to oocytes was competitively displaced by unlabeled IGF-1 or insulin with IC50 values of 6 and 560 nM, respectively. Chemical cross-linking of [125I]-IGF-1 to oocyte membranes specifically labeled a single 138-kDa protein. Using the technique of acid stripping to analyze whole-cell binding, both total and surface binding of [125I]IGF-1 reached a plateau after 90 min, and internalized counts surpassed surface-associated counts 20 min after hormone addition. At the 90-min plateau, 70-75% of cell-associated counts were internalized. The tyrosine kinase inhibitor, tyrphostin 47, prevented IGF-1-stimulated oocyte maturation with an apparent IC50 of 10.5 +/- 2.5 microM and also inhibited IGF-1 receptor-mediated endocytosis in micromolar concentrations. Treatment of oocyte membrane samples with 10 nM IGF-1 stimulated alkali-stable phosphorylation of a protein doublet (M(r) 89 and 104 kDa). Tyrphostin 47 also inhibited IGF-1-stimulated phosphorylation of these putative receptor beta-subunits more effectively than tyrphostin 23 or genistein. These results suggest a necessary role for receptor-mediated endocytosis in IGF-1-induced oocyte maturation and a requirement for receptor kinase activity in oocyte receptor internalization.
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