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  • Title: [Clonal analysis of the progeny of UV-irradiated Bacillus subtilis (uvr+ and uvr-) cells].
    Author: Lotareva OV, Filippov VD.
    Journal: Genetika; 1975; 11(12):146-9. PubMed ID: 817972.
    Abstract:
    The revertants to adenine prototrophy or mutants to auxotrophy can be easily identified on synthetic media which are partly enriched by caseine hydrolysate and yeast extract. It is shown with the use of these media that 1.5% colonies formed by Bacillus subtilis cells of the original type (ade6 met5) have mutant clones which are initiated by spontaneous revertants to adenine prototrophy. These revertants arise in the time of division of cells in macrocolonies. After plating diluted suspension of irradiated cells those colonies which contain mutant clones formed by spontaneous revertants can be erroneously taken for mixed colonies formed by induced revertants. About 40% mutants to auxotrophy induced by high dose of UV-light in in uvr+ cells form pure mutant colonies. The same mutants, induced by uvr cells by five time less UV-dose, usually form mixed colonies.
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